医学分子生物学杂志Issue(2)：63-68,6.DOI:10.3870/j.issn.1672-8009.2015.02.001
湖北地区119例 HCV 1b 型 NS3丝氨酸蛋白酶区的序列变异研究
Sequence Diversity of Hepatitis C Virus 1b NS3 Serine Protease Do-main in 119 Patients in Hubei Province of China
摘要
Abstract
Objective To analyze the sequence diversity of hepatitis C virus 1b NS3 serine protease domain in Chinese patients and study its influence on viral function and virus replica-tion. Methods Genotype-specific primers were designed according to the published sequences from database. Nested-PCR was used to amplify the NS3 region of 119 patients. PCR product was se-quenced. Homology analysis and phylogenetic tree were calculated by using the software. The muta-tion rate and variation of crucial functional amino acid were analyzed. Results Sequences in pa-tients from Hubei province of China shared a higher homology with HCV 1b standard strain and Asi-an sequences than the European and American sequences. The nucleotide and amino acid homology to 1b standard strain and Asian sequences ranged from 85. 94% to 87. 68% and 96. 83% to 92. 39% , respectively, and the nucleotide and amino acid homology to European and American se-quences was 85. 57% , and 92. 17% to 93. 38% , respectively. Phylogenetic analysis showed that the sequence from Hubei Province has a closer genetic distance to other published Chinese se-quences than sequences from Japan, Southeast Asia, Europe and America. There were 34 amino acid sites that had mutations within the 185 amino acids of serine protease region, but its important functional sites including the binding site for substrate of the catalytic enzyme and the Zn2 ﹢ binding sites were highly conserved in all sequences. Conclusion The analysis of the sequence diversity of HCV 1b NS3 serine protease domain and the biological significance enriches the understanding of variation and mutation of HCV 1b genome, and offers important molecular biology information for the study of HCV evolution in China.关键词
丙型肝炎病毒/NS3 区/序列分析Key words
hepatitis C virus/NS3/sequence analysis分类
生物科学引用本文复制引用
夏幼辰,杨东亮,潘雯,柯晓煜,王华,卢银平,郑昕,揭盛华,何生松,吴郡..湖北地区119例 HCV 1b 型 NS3丝氨酸蛋白酶区的序列变异研究[J].医学分子生物学杂志,2015,(2):63-68,6.基金项目
国家传染病科技重大专项课题(No.2008ZX10002011,2012ZX10004503),科技部国际合作与交流专项(No.2011DFA31030),华中科技大学研究生创新创业基金(No. HF-11-30-2013)This work was supported by grants from the National Major Science and Technology Special Project for Infectious Diseases of China (No.2008ZX10002011,2012ZX10004503), International Science & Technology Cooperation Program of China ( No.2011DFA31030) and Gradu-ates'Innovation Fund of HUST (No. HF-11-30-2013) （No.2008ZX10002011,2012ZX10004503）
