国际生殖健康/计划生育杂志Issue(3)：162-164,3.
辅助生殖技术治疗后自然流产胚胎的遗传学分析及其临床效应
Genetic Analysis and Its Clinical Effect of Spontaneous Abortion after Assisted Reproductive Techniques
摘要
Abstract
Objective:To analyze the chromosome abnormalities of spontaneous abortion tissues after assisted reproductive techniques (ART),and to discuss the relationship between genetic change and clinical feature. Methods:40 cases of spontaneous abortion villuses from ART, and 113 cases from natural pregnancy as control, were studied by the routine G banding karyotyping after the standard cell culture and harvest. Those cases of culture failure or without culture were analyzed by the multiplex ligation-dependent probe amplification (MLPA). Results: ①There was no significant difference in the rates of abnormal karyotyping between the ART group (50%, 20 cases) and the control group(53.1%,60 cases,P>0.05).②There were no significant differences in the rates of abnormal karyotyping when analyzed those parameters, including women age, primary or secondary infertility, infertility cause (male, female, or both), cycle type (fresh or frozen) (P>0.05). Although there was significant difference in this rate when analyzed IVF method (routine IVF and ICSI) in the limited cases(P<0.05), it should be further studied in more cases. Conclusions:Chromosome abnomalities are the main reason of spontaneous abortion after ART. The combined genetic techniques to detect the spontaneous abortion tissues are helpful for the ART treatment of infertility.关键词
生殖技术,辅助/流产,自然/绒毛膜绒毛/染色体/多重连接依赖的探针扩增技术Key words
Reproductive techniques,assisted/Abortion,spontaneous/Chorionic villi/Chromosomes/Multiplex ligation dependent probe amplification techniques引用本文复制引用
段程颖,李红,刘敏娟,陈瑛,孙健,丁扬,丁洁,王玮..辅助生殖技术治疗后自然流产胚胎的遗传学分析及其临床效应[J].国际生殖健康/计划生育杂志,2014,(3):162-164,3.基金项目
苏州市科技局社会发展项目（SS08019），江苏省“333工程”培养资金资助项目 （）
