国际口腔医学杂志Issue(1)：13-15,3.DOI:10.7518/gjkq.2014.01.003
云南白药对体外培养成骨细胞增殖分化的影响
Effects of Yunnan Baiyao on promoting osteoblast proliferation and differentiation in vitro
摘要
Abstract
Objective To investigate the effects of Yunnan Baiyao on promoting osteoblast proliferation and differentiation, and to provide theoretical basis for its bone repair mechanism. Methods Cranium was harvested from fetal SD rats. Then, osteoblast was acquired and cultured by repeating enzymatic digestion-adherent explant method identified via invert microscope, alkaline phosphorase stain, and stain of calcified nodules. After using different concentrations of Yunnan Baiyao stimulating osteoblasts, the proliferation of the acquired cells was examined through methyl thiazolyl tetrazolium assay. Alkaline phosphatase(ALP) activity and osteocalcin content were analyzed by using a spectrophotometer and enzyme linked immunosorbent assay in the cell lysate liquid. Results Compared with the controls, three different concentration groups can promote the proliferation of osteoblast; the 5 mg·L-1 group had more obvious roles in 5 and 7 d. It also increased the activity of ALP and promoted osteocalcin synthesis simultaneously. Conclusion Yunnan Baiyao can promote proliferation and differentiation of osteoblasts.关键词
云南白药/成骨细胞/碱性磷酸酶/骨钙蛋白Key words
Yunnan Baiyao/osteoblast/alkaline phosphatase/osteocalcin分类
医药卫生引用本文复制引用
任晓斌,和红兵,雷雅燕,张婉丽,张明珠..云南白药对体外培养成骨细胞增殖分化的影响[J].国际口腔医学杂志,2014,(1):13-15,3.基金项目
国家自然科学基金（81060086）；云南自然科学基金 （）
