国际口腔医学杂志Issue(4)：415-419,5.DOI:10.7518/gjkq.2015.04.010
高浓度葡萄糖对人牙周膜成纤维细胞成骨分化能力的影响
Effects of high glucose on osteogenic differentiation of periodontal ligament cellsin vitro
夏佳佳 1王岚 2章燕珍3
作者信息
- 1. 浙江大学医学院附属第二医院综合牙科 杭州 310000
- 2. 重庆医科大学附属口腔医院牙体牙髓病科，重庆市口腔疾病与生物医学重点实验室 重庆 401147
- 3. 浙江大学医学院附属第二医院口腔矫形科 杭州 310000
- 折叠
摘要
Abstract
Objective To isolate the periodontal ligament cells(PDLCs) and study the effect of high glucose concentration levels on the cellular activity and osteogenic differentiation of PDLCs from non-diabetic patients in vitro. Methods PDLCs were cultured for 14 days in 0 mg·L-1 glucose medium(C group), 1 100 mg·L-1 glucose medium(L group), and 4 500 mg·L-1 glucose medium(H group). Methyl thiazolyl tetrazolium(MTT) assay for cellular viability was performed. The osteogenic differentiation capacity of PDLCs was evaluated by alizarin red staining and alkaline phosphatase(ALP) activity assaying and real time-transcription polymerase chain reaction. Results H group significantly inhibited the proliferation of PDLCs and reduced the optic density of the MTT and ALP activity assay. The difference was significant(P<0.05). Concerning the mineralized nodule formation, the percentage of the calcified area and the expression of osteogenic genes(ALP, Runx-2, Col-Ⅰ) to the total culture dish of PDLCs in the H group were significantly lower than that in the L and C groups(P<0.05). Conclusion High glucose inhibits the proliferation and differentiation of PDLCs.关键词
葡萄糖/牙周膜成纤维细胞/成骨诱导Key words
glucose/periodontal ligament cell/osteogenic differentiation分类
医药卫生引用本文复制引用
夏佳佳,王岚,章燕珍..高浓度葡萄糖对人牙周膜成纤维细胞成骨分化能力的影响[J].国际口腔医学杂志,2015,(4):415-419,5.
