临床与病理杂志Issue(5)：834-839,6.DOI:10.3978/j.issn.2095-6959.2015.05.028
抑制survivin基因的表达对骨肉瘤细胞株U-2OS凋亡的影响
Blockade of survivin promotes the apoptosis of human osteosarcoma cell line U-2OS
摘要
Abstract
Objective:To investigate the effect of surviving on the apoptosis of human osteosarcoma cell line U-2OS. Methods:Atfer transfection with small inference RNA targeting survivin in U-2OS, survivin protein and mRNA expression were measured with western blot and RT-PCR, respectively. U-2OS cells were knockdown with survivin, and then were incubated with cisplatin for the indicated times, cell survival rate, growth inhibitory rate and apoptosis rate were measured with MTT and lfow cytometry, respective;Bcl-2 and Bax protein expressions were measured with western blot. Results:Knockdown of survivin significantly decreased the protein and mRNA expression of survivin (P<0.05). Furthermore, survivin deifciency dramatically augmented the effect of cisplatin on cell survival rate, growth inhibitory rate, apoptosis rate, and the expression of Bcl-2 and Bax (P<0.05).Conclusion:Absence of survivin accelerated the apoptosis of U-2OS, and increased the chemosensitivity of U-2OS towards cisplatin;the present study reveals a novel role of surviving in the treatment of osteosarcoma.关键词
survivin/siRNA/肉骨瘤细胞株U-2OS/化疗敏感性Key words
survivin/siRNA/osteosarcoma cell line U-2OS/chemosensitivity引用本文复制引用
张文韬,段宁,程辉光,宋涛,杨团民,同志超..抑制survivin基因的表达对骨肉瘤细胞株U-2OS凋亡的影响[J].临床与病理杂志,2015,(5):834-839,6.基金项目
西安交通大学医学院附属红会医院基金(YJ2013012)。Tis work was supported by Red Cross Hospital Afliated to Xi'an Jiaotong University Fund (YJ2013012), P. R. China （YJ2013012）
