神经损伤与功能重建Issue(5)：369-372,4.DOI:10.3870/sjsscj.2014.05.003
MRS2179对大鼠缺血性脑卒中后MMP-9表达及血脑屏障通透性的影响
MRS2179 Reduces the MMP-9 Expression and Blood-brain Barrier Permeability in Rat Brain after Focal Cerebral Ischemia
摘要
Abstract
Objective:To observe the effects of selective P2Y1R antagonist, MRS2179, on the expression of MMP-9 and the blood-brain barrier (BBB) permeability in a rat model of middle cerebral artery occlusion(MCAO). Methods:All 45 SD rats were randomized into 3 groups:sham group (n=15), model group (n=15)and MRS2179 group (n=15). Intracerebroventricular administration of 5 μL vehicle control (aCSF) was performed to the sham group and the model group. Meanwhile, 5μL of MRS2179 (2 nmol) was given to the rats in the MRS2179 group in the same way. The MCAO models were made in the groups control and MRS2179.The sham group was treated in the same manner as those undergoing MCAO, however, no occluding thread was inserted. After 24 h reperfu-sion, the wet/dry method was used to evaluate the brain water content of the ischemic hemisphere, the Evans Blue dye (EB) extravasation was measured to assess the BBB permeability and the expression of MMP-9 was deter-mined by Western blot. Results:The brain water content in the model group, the sham group and the MRS2179 group was (81.72±0.23)%, (77.65±0.27)%, (79.92±0.24)%respectively(n=6). Compared with the model group, the brain water content in the MRS2179 group was decreased, but was still higher than that in the sham group ( <0.01) .The EB extravasation from the ischemic hemisphere in the model group was (1737.60±67.46)ng/g, remarkably higher than those in the in the MRS2179 group [(1165.15±93.26)ng/g] and sham group [(324.18±63.35)ng/g] ( <0.01) (n=6). In terms of the expression of MMP-9 in the ischemic penumbra, the protein amount was (0.52±0.05), (1.69±0.08) and (0.99±0.10) respectively in the sham group, the model group and the MRS2179 group (n=3). The protein amount in the MRS2179 group was significantly lower than that in the model group ( <0.01). Conclusion:Inhibition of P2Y1R by MRS2179 might reduce the BBB permeability and relieve brain edema after the ischemic cerebral stroke by decreasing the expression of MMP-9.关键词
MRS2179/缺血性脑卒中/血脑屏障/脑水肿/基质金属蛋白酶-9Key words
MRS2179/ischemic cerebral stroke/blood-brain barrier/brain edema/matrix metalloproteinase-9分类
医药卫生引用本文复制引用
张秋佳,杨锦珊,刘阳,王伟..MRS2179对大鼠缺血性脑卒中后MMP-9表达及血脑屏障通透性的影响[J].神经损伤与功能重建,2014,(5):369-372,4.基金项目
国家自然科学基金 （）
