神经损伤与功能重建Issue(2)：134-137,4.DOI:10.3870/sjsscj.2015.02.013
胸腺基质淋巴细胞生成素诱导重症肌无力患者胸腺 CD4+CD25－T 细胞向 CD4+CD25+Foxp3+调节性T 细胞分化
Differentiation of Intrathymic CD4 +CD25 - T Cells to CD4 +CD25 +Foxp3 + Regulatory T Cells In-duced by Thymic Stromal Lymphopoietin in Myasthenia Gravis Patients
摘要
Abstract
Objective: To investigate the effect of thymic stromal lymphopoietin (TSLP) on the differentiation of intrathymic CD4+CD25- T cells to CD4+CD25+Foxp3+ regulatory T cells in myasthenia gravis (MG) patients with abnormal thymus gland. Methods: Intrathymic CD4+CD25-T cells and dendritic cells isolated by immunomagnetic beads on MiniMACS device were co-cultured with different concentrations of TSLP (0.1, 1, 10, 20 ng/mL) for 7 days. The ratio of CD4+CD25+Foxp3+Treg/CD4+T cells was tested by flow cytometry. The expression of Foxp3 mRNA and protein was measured by RT-PCR and Western blot respectively. The concentration of serum IL-10 was detected by ELISA. Results: The ratio of CD4+CD25+Foxp3+Treg/CD4+T cell, the expression of Foxp3 mRNA and protein, and the level of IL-10 was increased significantly in groups with 10 ng/mL and 20 ng/mL TSLP compared with those in the PBS control group (P<0.05). Conclusion: TSLP may induce the differentiation of intrathymic CD4+CD25 -T cells to CD4+CD25+Foxp3+regulatory T cells in MG patients with abnormal thymus gland and up-regulate the expression of Foxp3.关键词
重症肌无力/胸腺基质淋巴细胞生成素/调节性 T 细胞Key words
myasthenia gravis/thymic stromal lymphopoietin/regulatory T cells分类
医药卫生引用本文复制引用
卢祖能,袁江,张跃亮,王云甫..胸腺基质淋巴细胞生成素诱导重症肌无力患者胸腺 CD4+CD25－T 细胞向 CD4+CD25+Foxp3+调节性T 细胞分化[J].神经损伤与功能重建,2015,(2):134-137,4.基金项目
湖北省教育厅重点项目（No.D20112102）；湖北省科技厅自然科学基金 （）
