高校化学工程学报Issue(3)：600-605,6.DOI:10.3969/j.issn.1003-9015.2014.03.026
以甘油为碳源分批补料高密度发酵产环氧化物水解酶
Glycerol as a Carbon Source to Produce Epoxide Hydrolase by High Cell Density Fed-Batch Cultivation
摘要
Abstract
Epoxide hydrolase is an important industrial enzyme which can stereo-selectively transform racemic epoxide into enantiomeric vicinal diol. In this study, the growth of E. coli Rosetta (DE3)/pET28(a)-CESH on different concentrations of glycerol was studied in shaking flask. Induction time, IPTG concentration and culture temperature after induction were optimized to enhance the production of CESH. The results reveal that in a 15 L fermentor, the fed-batch fermentation process can reduce the fermentation time by 10 hours. Compared with the batch fermentation process, the CESH productivity was increased by 33.3%during the whole process. These results indicate that the new process is suitable for industry application.关键词
重组大肠杆菌/环氧化物水解酶/高密度发酵/甘油抑制Key words
recombinant E. coli/epoxide hydrolase/high cell density cultivation/glycerol inhibition分类
化学化工引用本文复制引用
陈冠宇,袁建锋,吴绵斌,林建平,杨立荣..以甘油为碳源分批补料高密度发酵产环氧化物水解酶[J].高校化学工程学报,2014,(3):600-605,6.基金项目
国家科技部863项目(2014AA022105)。 （2014AA022105）
