高校化学工程学报Issue(4)：934-938,5.DOI:10.3969/j.issn.1003-9015.2014.04.039
汽爆玉米秸秆发酵丁醇的酶解工艺优化
Optimization of Enzymatic Hydrolysis Conditions of Steam-Exploded Corn Stover
摘要
Abstract
Influence of different factors such as temperature, original substrate concentration, enzyme loading, hydrolysis time, and stirring rate on steam-exploded corn stover enzymolysis were studied to further improve reducing sugar concentration and butanol yield after reaction. It is found that the optimal hydrolysis reaction condition is:temperature 50℃, 100 r⋅min-1, enzyme loadings 60 IU/(g corn stover), solid:liquid ratio 3:10 and reaction time 48 h. Reducing Sugar concentrations were tested with DNS(3,5-Dinitrosalicylic acid) and butanol yield was tested with gas chromatographic methods. Moreover, the highest apparent viscosity and apparent viscosity of a 3-step loading are lower than those of 2-step loading. However, the sugar concentration is slightly higher than that of the later one. When the substrate with original concentration of 15%(w/w) was added with to final concentration of 25% (w/w) by 3 steps, the reducing sugar concentration and butanol yield reach to 89.23 mg⋅mL-1, 9.82 mg⋅mL-1, respectively, which show an increase of 58.63%, 44.20%, respectively.关键词
丁醇/发酵/玉米秸秆/蒸汽爆破/酶水解/表观黏度Key words
butanol/fermentation/corn stover/steam-exploded/enzymatic hydrolysis/apparent viscosity分类
化学化工引用本文复制引用
张霞,李红伟,马晓建..汽爆玉米秸秆发酵丁醇的酶解工艺优化[J].高校化学工程学报,2014,(4):934-938,5.基金项目
国家十一五科技支撑计划(2007BAD66B04)。 （2007BAD66B04）
