高校化学工程学报Issue(5)：1051-1058,8.DOI:10.3969/j.issn.1003-9015.2014.05.14.05
C-G-Ha-HS支架改性协同原儿茶酸对NS/PCs粘附及定向分化的影响
The Synergistic Effect of Modified Chitosan-Gelatin-Hyaluronate-Heparan Sulfate Scaffolds and PCA on NS/PCs Adhesion and Differentiation
摘要
Abstract
Chitosan-gelatin-hyaluronate-heparan sulfate (C-G-Ha-HS) scaffolds were fabricated and modified with laminin (LN) in order to improve scaffold adhesion and optimize 3-D culture system of neural stem/progenitor cells (NS/PCs). The effects of protocatechuic acid (PCA) on the inducing of NS/PCs differentiation into dopaminergic neuron were determined at 3-D level. SEM and CCK-8 assay results show that the pore size of the C-G-Ha-HS scaffolds is 90~130 µm and the NS/PCs are connected with the scaffolds to form reticular structure. Compared with LN-unmodified C-G-Ha-HS(5:5) scaffolds, LN-modified scaffolds enhance the NS/PCs adhesion rate by (113.53 ± 4.32)%after 4 hours and increase cell viability by (120.30 ± 6.65)%after 96 hours. Immunocytochemistry assay shows that the synergistic effect of 1%Fetal Bovine Serum (FBS) and 0.06 mmol⋅L-1 PCA can enhance the differentiation rate of NS/PCs into neurons. The differentiation rate of TH and Nurr1 positive cell can reach up to (16.53 ± 0.65)% and (15.93 ± 0.91)%, respectively. Compared with the control scaffold group, the differentiation rate of TH and Nurr1 positive cell are enhanced by (172.72 ± 6.79)%and (181.23 ± 1.04)%. These results provide experimental data for the application of PCA in the treatment of PD by NS/PCs transplantation.关键词
壳聚糖-明胶-透明质酸-硫酸肝素支架/层粘连蛋白/神经干/祖细胞/原儿茶酸/分化Key words
chitosan-gelatin-hyaluronate-heparan sulfate scaffold/laminin/neural stem/progenitor cells/protocatechuic acid/differentiation分类
生物科学引用本文复制引用
张晓庆,关水,葛丹,王树萍,刘天庆,马学虎..C-G-Ha-HS支架改性协同原儿茶酸对NS/PCs粘附及定向分化的影响[J].高校化学工程学报,2014,(5):1051-1058,8.基金项目
国家自然科学基金(30800288) （30800288）
中央高校基本科研业务费专项资金资助项目(DUT12JB04)。 （DUT12JB04）
