高校化学工程学报Issue(2)：351-357,7.DOI:10.3969/j.issn.1003-9015.2015.02.016
微生物燃料电池中Tolumonas Osonensis菌株的产电性能优化
Optimization of Power Generation Performance ofTolumonas Osonensis in MFCs
摘要
Abstract
Power generation performance of Tolumonas osonensis P2-A-1 in MFCs was improved by optimizing initial cell concentration in inoculation medium, substrate type and concentration, anolyte pH, external resistance, and chemical solvent permeabilization. The optimized experimental condition is inoculation in TSB liquid medium and statically incubated at 22℃ under aerobic conditions. OD600 of the culture was 2.714 after 48 h of incubation and cells were harvested by centrifugation and treated by 1 mmol⋅L−1 EDTA for 20 min. The cells were added into anolyteⅡ (PBBM liquid medium containing 2.000 g⋅L−1 sodium acetate as the substrate and 5.000 g⋅L−1 ferric citrate, pH 7.2) to prepare inoculation media with dry cell concentration of 3.394 g⋅L−1. After 14 d at room temperature, the power density of MFCs connecting to the external resistance 1000Ω reaches to 509 mW⋅m−2 , which is increased by 162.4% compared with the original. It is the first time thatT. osonensis is applied in MFCs and shows electrochemical activity.关键词
微生物燃料电池/Tolumonas osonensis/电化学活性/产电条件优化/细胞通透化Key words
microbial fuel cells/Tolumonas osonensis/electrochemical activity/operating conditions/optimization/cell permeabilization分类
化学化工引用本文复制引用
杨佳,李明,朱思远,骆健美,周明华,王敏..微生物燃料电池中Tolumonas Osonensis菌株的产电性能优化[J].高校化学工程学报,2015,(2):351-357,7.基金项目
国家自然科学基金(21328602)；天津科技大学大学生实验室创新基金项目(1404A203)；农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室开放基金(2012-SZJJ-CYM)。 （21328602）
