广西科学Issue(2)：108-114,7.
酿酒酵母YBR019C 基因缺失突变的分析
Reseach on Saccharomyces cerevisiae Mutant Deficient inYBR019C
摘要
Abstract
[Objective]Our study focuses on the saccharometabolism and ethanol fermentation of Saccharomyces cerevisiae strain NF1002.[Methods]YBR 019C on Chromosome Ⅱ was chosen to be modified by PCR with pUG6 and pUG66 plasmids as templates.After homologous recom-bination of Cre/loxP mediated marker and YBR 019C,YBR 019C deficient mutant of Saccharomyces cerevisiae strain NF1002 was constructed.[Results]Glucose and sucrose can be utilized for metabolism at both mutant and wild strains except lactose and xylose.However,on-ly part of raffinose and maltose and few galactose can be utilized at the mutant strain.Results of sucrose fermentation at mutant strain NF-ybr displayed that ethanol content reached 13.68%(V/V)at the end of fermentation,which was 10. 7% higher than that for the wild strain.Further-more,according to the industrial processes for ethanol fermentation of sugarcan molasses,etha-nol content was 12.015%(V/V)at 30℃ for 72h, lower than that of the wild strain.[Conclusion]Saccharometabolism ofSaccharomyces cerevisiae strain NF1002 was affected by deletion of gene YBR 019C ,showing less ethanol production.Our research provided advice on modification of Saccharomyces cerevisiae strains on an efficient way.关键词
酿酒酵母/YBR 019C/基因敲除/甘蔗糖蜜/乙醇Key words
Saccharomyces cerevisiae/YBR 019C/gene deletion/sugarcan molasses/ethanol分类
生物科学引用本文复制引用
黄艳燕,郭铃,陈东,龙思宇,李检秀,陆琦,孙靓,黄日波..酿酒酵母YBR019C 基因缺失突变的分析[J].广西科学,2014,(2):108-114,7.基金项目
广西科学院基本科研业务项目（基金编号11YJ24SW06）；八桂学者建设工程专项经费项目资助。 （）
