广西科学Issue(5)：541-549,9.
广西药用植物两面针遗传多样性的ISSR分析
ISSR Analysis on Genetic Diversity of Medically Impor-tant Zanthoxylum nitidum (Roxb.)DC.in Guangxi
摘要
Abstract
Objective]Zanthoxylum nitidum (Rutaceae)is an important medicinal plant mainly distributed in southern China.In recent years,however,the distribution range of this species has been reduced greatly due to human over exploitation and deterioration of its habitat.Stud-ying the population genetics of this species is essential for the conservation and sustainable uti-lization of the plant germplasm resource.[Methods]In this study,we used eight ISSR markers to measure genetic diversity of 138 individuals from eleven populations of Z.nitidum in Guan-gxi.[Results]A total of 63 DNA fragments were scored,and the percentage of polymorphic bands (P )was 100%.High level of genetic diversity was detected inZ.nitidum (He=0.227;I=0.356).AMOVA analysis indicated that coefficient of genetic differentiation(Gst)was 0.490 (51% of the total genetic variation resided within a population and 49% between popula-tions),which demonstrated that the genetic variation between populations and within a popula-tion was roughly equal.Gene flow (Nm)between each population was 0.52,which showed a relatively lacking gene flowing.The genetic distance between each population ranged 0.053~0.334.The results of Mantel test (r=0.391,p=0.040)and UPGMA cluster indicated that there was no significant relationship between geographical distance and genetic distance.[Con-clusion]This investigation indicats that the ge-netic diversity of Z.nitidum in Guangxi is high, which is good for the cultivation of high quality herbmedicine;In situ conservation is advocated first,and keeping a stable environment for this herb is necessary.关键词
药用植物/两面针/遗传多样性/ISSRKey words
medicinal plants/Zanthoxylum nitidum/genetic diversity/ISSR分类
农业科技引用本文复制引用
向巧彦,黄夕洋,李虹,蒋水元..广西药用植物两面针遗传多样性的ISSR分析[J].广西科学,2014,(5):541-549,9.基金项目
广西科学研究与技术计划项目(桂科攻1099063-10),横向项目“两面针病虫害防治技术研究”和“两面针规范化种植技术研究”,广西科学院科技创新项目(2010ZW002)资助。 （桂科攻1099063-10）
