广西科学Issue(6)：619-623,633,6.
发酵耦合酶解高效制备右旋糖酐工艺研究
Study on Efficient Preparation for Dextran by Fermen-tation Coupled with Enzymatic Hydrolysis
摘要
Abstract
Objective]This paper aims to control dextran molecular flexibly and prepare dextran efficiently with Leuconostoc mesenteriodes 31208 by improving the preparation process.[Meth-ods]The effect of sucrose concentration on sucrose conversion,the parameters of sucrose feed-ing,as well as the process conditions coupled enzymatic adding and sucrose feeding were stud-ied by means of single factor experiment.[Results]The molecular weight of dextran was con-trolled flexibly by changing the enzymatic hydrolysis process,and the parameters of optimal su-crose feeding process were the initial sucrose concentration of 130 g/L with the flow accelera-tion of 12 g·L-1 ·h-1 at 16 h.Compared with the single batch fermentation,the conversion rate improved by 75% and specific-molecular dextran(5000~7500 Da)increased over time,and the yield of dextran was about 32%.[Conclu-sion]This method has the characteristics of sim-ple fermentation control,high efficiency and low cost,which was important to the preparation of dextran for industrial production.关键词
右旋糖酐/酶法/右旋糖酐酶/流加Key words
dextran/enzymic method/dextranase/feeding分类
化学化工引用本文复制引用
常国炜,梁达奉,张九花,曾练强,谭文兴,黎志德..发酵耦合酶解高效制备右旋糖酐工艺研究[J].广西科学,2014,(6):619-623,633,6.基金项目
国家863计划项目（2012AA021504）和八桂学者建设工程专项经费项目资助。 （）
