广西科学Issue(3)：341-345,5.
小月柳珊瑚中生物碱类化学成分及其抗骨肉瘤细胞增殖活性研究
Chemical Compositions of Alkaloids from Marine Gor-gonian Menella kanisa and Their activities of Anti-pro-liferation on Human Osteosarcoma Cell U2 OS
摘要
Abstract
[Objective]The alkaloids from marine gorgonian Menella kanisa and their activities of anti-proliferation on U2 OS cancer cell were investigated.[Methods]The alkaloids were isolated and purified by column chromatography on silica gel,and their structures were identified by spectral analyses and compared with literatures. The cytotoxicity of compounds 1 ~2 toward hu﹣man osteosarcoma cell U2 OS cancer cell line was evaluated by MTT methods.[Results]Seven al﹣kaloids were obtained from M.kanisa and char﹣acterized as 6-(1ˊ-purine-6ˊ,8ˊ-dionyl)suberos﹣anone (1),1-(3ˊ-carbonylbutyl)purine-6,8-dione (2),adenine deoxyribonucleoside (3),hypoxanthine (4),7-methyladenine (5),thymine de﹣oxyribonucleoside (6)and uracil deoxyriboside (7).Compounds 1 and 2 showed moderate cyto﹣toxicity against osteosarcoma U2 OS cell line with IC50 of 13.3 and 55.0 μg/mL,respectively.[Conclusion]Compounds 1 ~ 7 were isolated from gorgonian M.kanisa for the first time.The antitumor activity of Compounds 1 and 2 against U2 OS in vitro was also reported for the first time.关键词
小月柳珊瑚/生物碱/结构鉴定/抗肿瘤细胞活性Key words
Menella kanisa/alkaloid/chemical identification/anticancer分类
医药卫生引用本文复制引用
姚金娥,杨渊,孙海燕,唐林,巫繁菁,韦玮,黄慧学,高程海..小月柳珊瑚中生物碱类化学成分及其抗骨肉瘤细胞增殖活性研究[J].广西科学,2015,(3):341-345,5.基金项目
广西科技攻关项目(桂科攻1140003B),三亚市院地科技合作项目(2013YD73),广西自然科学基金项目(2011GXNSFB018077)资助。 （桂科攻1140003B）
