南方农业学报Issue(2)：265-270,6.DOI:10.3969/jissn.2095-1191.2015.2.265
深绿木霉SS003产胞壁降解酶发酵条件的响应面优化
Optimizing fermentation conditions for cell wall degrading enzymatic production from Trichoderma atroviride SS003 by response surface method
摘要
Abstract
Objective]The fermentation conditions of β-glucanase and chitinase production from T. atroviride were optimized to provide references for the research on its biocontrol mechanism. [Method]Three independent variables were selected for single factor test , which were incubation time ( 24 , 48 , 72 , 96 , 120 , 144 , 168 hours ) , culture temperature ( 24 , 26 , 28 , 30 , 32 , 34 ℃) and initial inoculums size ( 3 , 4 , 5 , 6 , 7 , 8 , 9 pieces ) . Based on single factor optimization results , response surface method was used to optimize the above conditions in line with Box-Behnken principle of De-sign-Expert 8.05 b. [Result]The optimum conditions for β-glucanase were obtained which were incubation time of 73.70 hours, culture temperature at 27.82 ℃ and initial inoculums size of 5.19 pieces. For chitinase, the optimum conditions were incubation time of 82.91 hours, culture temperature at 27.70 ℃ and initial inoculums size of 5.03 pieces. [Con-clusion]Due to its effectiveness in boosting quantity of β-glucanase and chitinase from Trichoderma atroviride SS003 , response surface method should be employed to optimize the fermentation conditions for cell wall degrading enzymatic production.关键词
深绿木霉/β-葡聚糖酶/几丁质酶/响应面法Key words
Trichoderma atroviride/β-glucanase/chitinase/response surface分类
生物科学引用本文复制引用
鲍泽然,敖新宇,陈涛,陈玉惠..深绿木霉SS003产胞壁降解酶发酵条件的响应面优化[J].南方农业学报,2015,(2):265-270,6.基金项目
国家自然科学基金项目 （）
