南方农业学报Issue(2)：303-307,5.DOI:10.3969/jissn.2095-1191.2015.2.303
微波消解-紫外分光光度法测定三叶青总游离氨基酸
Determination of total free-amino acid in Tetrastigmatis hemsleyani by microwave digestion-UV spectrophotometer
摘要
Abstract
Objective]The best method of determining the total free-amino acid in Tetrastigmatis hemsleyani was explored to provide the scientific evidence for its comprehensive development and utilization. [Method]Tetrastigmatis hemsleyani was used as the material and aspartic acid as the standard. The microwave digestion extraction technology of total free-amino acid from Tetrastigmatis hemsleyani was optimized by orthogonal experiments , and its content was de-termined by UV spectrophotometer. [Result]The descending sequence of influencing microwave digestion extracting the total free-amino acid was material - liquid ratio, extracting temperature and extracting power, extracting time. The op-timum extraction process was as follows: using distilled water as solvent , under 225 W and 60 ℃ for an extraction of 20 min with the material-liquid ratio of 1∶15. Under the best processing condition, the average content total free-amino acid was 0.678%. The best wavelength to determine aspartic acid standard solution and the Tetrastigmatis hemsleyani test solution was 565 nm. Aspartic acid showed a better linearity and good correlation within a range of 0.001-0.014 mg/mL, R2=0.9991. [Conclusion]Microwave digestion-UV spectrophotometer is effective and simply operative with less solvent and shorter extraction time.关键词
三叶青/总游离氨基酸/微波消解-紫外分光光度法/正交试验/含量测定Key words
Tetrastigmatis hemsleyani/total free-amino acid/microwave digestion-UV spectrophotometer/orthogonal test/content determination分类
医药卫生引用本文复制引用
付金娥,韦树根,冀晓雯,童婷婷,莫长明..微波消解-紫外分光光度法测定三叶青总游离氨基酸[J].南方农业学报,2015,(2):303-307,5.基金项目
广西卫生厅重点项目（重2012116）；南宁市科学研究与技术开发计划项目 （）
