南方农业学报Issue(2)：332-337,6.DOI:10.3969/jissn.2095-1191.2015.2.332
杂交鳢海豚链球菌病病原分离鉴定及药敏试验
Isolation and identification of pathogen Streptococcus iniae in diseased hybrid snakehead (Channa argus♂× Channamaculata♀)
摘要
Abstract
Objective]The pathogens from diseased hybrid snakehead (Channa argus♂×Channa maculata♀) in an aquafarm in Zhongshan City of Guangdong Province were isolated and identified and antibiotic sensitivity test was per-formed in order to determine the causes and provide references for preventing and controlling this disease effectively.[Method]Pathogenic bacteria were isolated from moribund hybrid snakehead , and they were identified through physio-logical and biochemical characteristics test and 16S rRNA sequence analysis. Furthermore , antibiotic sensitivity test was performed through Kirby-Bauer disk diffusion method. [Result]Two dominant bacterias (ZS2013711-1, ZS2013711-2) were isolated from moribund hybrid snakehead , and they were identified to be Streptococcus iniae according to their physiological and biochemical characteristics and 16S rRNA sequence analysis. The half lethal dose values for the iso-lates to healthy hybrid snakehead were 2.7 ×105 and 6.7 ×105 CFU/g, respectively. The results of antibiotic sensitivity test showed the isolates were highly sensitive to cefotaxime, cefradine, cephazolin, lincomycin, and so on. [Conclu-sion]Hybrid snakehead is a new found host for S. iniae. In hybrid snakehead aquaculture industry , its disease resulted from S. iniae can be prevented and treated by cefotaxime, cefradine, cephazolin and lincomycin.关键词
杂交鳢/海豚链球菌/分离/鉴定/药敏试验Key words
hybrid snakehead/S. iniae/isolation/identification/antibiotic sensitivity test分类
农业科技引用本文复制引用
陈言峰,周爱国,邹青,陈冠锋,陈建酬,张继平,张辉华..杂交鳢海豚链球菌病病原分离鉴定及药敏试验[J].南方农业学报,2015,(2):332-337,6.基金项目
广东省教育部产学研合作项目（2010b090400255）；广东省普通高校青年创新人才项目（2014KQNCX183）；佛山科学技术学院科研项目 （）
