广西师范大学学报（自然科学版）Issue(3)：109-115,7.
水稻细菌性条斑病菌一个假定的udgH基因的功能研究
Functional Analysis of a Hypothetical udg H Gene in Xanthomona oryzae pv.oryzicola
摘要
Abstract
Xanthomonas oryzae pv.oryzicola (Xoc)is an important plant pathogen,which caused rice bacterial leaf blight and seriously decreased the production of rice.A mutation in putative udg H gene of Xoc leads to serious weakening on extracellular polysaccharide production and virulence,and lower ex-tracellular amylase and cellulase activity.The phenotype of complementary strain can be restored to wild-type levels,while virulence only by 80%,and the extracellular polysaccharide production of high expression strain is increased,while the virulence is decreased.The research laid solid foundation for further study the role of the gene in extracellular polysaccharide metabolic pathways of Xoc,and provid-ed a feasible method for constructing high-yielding strains of xanthan gum useing genetic engineering.关键词
水稻细菌性条斑病菌/UDP-脱氢酶基因/Tn5 插入突变体/胞外多糖/致病力Key words
Xanthomonas oryzae pv.oryzicola/UDP-dehydrogenase gene/Tn5-tagged mutant/extracel-lular polysaccharide/virulence分类
生物科学引用本文复制引用
蔡文侠,黄光辉,王婷婷,付珊,何勇强,姜伟..水稻细菌性条斑病菌一个假定的udgH基因的功能研究[J].广西师范大学学报（自然科学版）,2014,(3):109-115,7.基金项目
国家自然科学基金资助项目(31260261、31360021) （31260261、31360021）
广西自然科学基金资助项目(20130231) （20130231）
广西教育厅基金资助项目(20130300) （20130300）
