广西医科大学学报Issue(1)：5-7,3.
慢病毒介导基因转染人韧带成纤维细胞表达的研究
EXPRESSION OF GREEN FLUORESCENT PROTEIN GENE IN HUMAN LIGAMENT FIBRO-BLASTS MEDIATED BY LENTIVIRAL VECTOR
摘要
Abstract
Objective:To explore the transfection and expression of green fluorescence protein reporter gene (GFP)in Human ligament fibroblasts mediated by lentivirus.And whether affect biophysical properties of ligament fibroblasts and provide theoretical basis for the next experiment.Methods:Human ligament fibro-blasts were Isolated and cultured by Tissue-adherent method and were transfected with lentiviral vector car-rying GFP.The GFP expression of ligament fibroblasts was observed under a fluorescence microscopy after 9 6 h.Growth curve was acquired by MTT and FACS to compare the difference of the growth rate between experimental and control groups.Results:Under the fluorescence microscope,green fluorescent was found partly after 9 6 h.And the GFP positive expression rate (transfection efficiency)was 9 5%.Flow cytometry testing results displayed that apoptosis of fibroblasts infected with GFP lentivirus were unaffected,as com-paredwith control MSC(P >0.05).The result of MTT measured growth curve showed that there fibro-blasts was no significant difference in the proliferative capability of chondrocytes between the experimental and control groups.Conclusion:The lentiviral vector containing green fluorescence protein reporter gene was infected Human ligament fibroblasts efficiently and expressed stably,which will provide a reliable tracer method,a basis for gene modification of ligament fibroblasts through the lentiviral vector.关键词
慢病毒载体/人韧带成纤维细胞/绿色荧光蛋白Key words
lentiviral vector/human ligament fibroblasts/green fluorescence protein分类
医药卫生引用本文复制引用
刘锦愉,韩创业,赵春节,陈首,杨劲松..慢病毒介导基因转染人韧带成纤维细胞表达的研究[J].广西医科大学学报,2015,(1):5-7,3.基金项目
国家自然科学基金地区科学基金项目(No.81160217) （No.81160217）
