广西医科大学学报Issue(1)：8-10,3.
鸡整胚原位杂交RNA探针的制备
THE METHOD FOR WHOLE-MOUNT IN SITU HYBRIDIZA-TION OF CHICK EMBRYOS WITH RNA PROBE
摘要
Abstract
Objective:To develop a fast,simple and efficient protocol for whole-mount in situ hybridization of chick embryos with digoxigenin(DIG)-Labeled RNA probe.Methods:The target fragment of gene was ob-tained by RT-PCR through total RNA of chicken,using the RNA polymerase promoter,based on linear DNA template in vitro transcribed into DIG-Labeled RNA probe.Results:Obtained the DIG-Labeled RNA probe and positive signals were viewed in chick embryos.Conclusion:The successful preparation of DIG-La-beled RNA probes lays an experimental foundation for whole-mount in situ hybridizatation of chick embry-os which be used as an animal mode.关键词
鸡胚/RNA探针/整胚原位杂交/地高辛Key words
chick embryos/RNA probe/whole-mount in situ hybridization/digoxigenin分类
生物科学引用本文复制引用
陈丽,陀杨娟,孙晋虎..鸡整胚原位杂交RNA探针的制备[J].广西医科大学学报,2015,(1):8-10,3.基金项目
国家自然科学基金资助项目(No.31060167) （No.31060167）
