广西医学Issue(4)：409-412,4.DOI:10.11675/j.issn.0253-4304.2014.04.01
小鼠鼻气道阻力检测方法的建立
New Method for Measurement of Nasal Airway Resistance in Mice
摘要
Abstract
Objective To introduce a simple and available method for measurement of nasal airway resistance ( RNA) and nasal lavage in mice .Methods Sixteen female mice were randomly divided into 2 groups:allergic rhinitis (AR) group and control group ,with 8 mice in each group.AR mice were immunized by intraperitoneal injection of ovalbumin plus suspension of aluminum hydroxide and normal saline as well as challenged with intranasal ovalbumin plus the suspension above .Control mice were sensitized by systemic intraperitoneal injection of normal saline and then challenged with intranasal normal saline .All mice were anesthetized after challenge .A cannula (18G) was inserted into the trachea .A blunt needle connected with a T tube was inserted into nasopharynx .One end of the T tube was connected to a syringe pump which provided consistent air flow to the nasal passages .Another end of the T tube was connected to a pressure transducer for measurement of pressure difference across nasal passage .Pressure difference was divided by air flow to give values of RNA .Histamine was nebulized into nasal cavity to measure nasal response .A polyethylene catheter connected to a syringe was gently inserted into one nostril and the fluid was simultaneously collected from the contralateral nostril.Nasal lavage cytology and nasal histology were measured .Results The basic and postchallenge RNA values as well as nasal response values for AR group were higher than those of control group (allP<0. 01) .Eosinophils and neutrophils in nasal lavage fluid increased significantly in the AR group compared with the controls (all P<0 .01) .Obvious infiltration of eosinophils and mild edema were observed in the nasal mucosa in the AR group , but weren't observed in the control group .Conclusion We establish a relatively simple and available method for measuring nasal airway resistance and nasal lavage in mice .关键词
应变性鼻炎/鼻气道阻力/鼻灌洗/小鼠Key words
Allergic rhinitis/Nasal airway resistance/Nasal lavage/Mouse分类
医药卫生引用本文复制引用
谢佳星,张清玲,罗炜,赖克方..小鼠鼻气道阻力检测方法的建立[J].广西医学,2014,(4):409-412,4.基金项目
国家自然科学基金资助项目 （）
