广西医学Issue(4)：420-423,4.DOI:10.11675/j.issn.0253-4304.2014.04.04
转化生长因子-β1与系统性硬化病-肺纤维化相关性的实验研究
Correlation between Transforming Growth Factor -β1 and Systemic Sclerosis-Pulmonary Fibrosis
摘要
Abstract
Objective To investigate transforming growth factor-β1( TGF-β1) expression in the model mice with systemic sclerosis-pulmonary fibrosis(SSc-PF) and its correlation with SSc-PF.Methods Thirty male mice were randomly divided into Group A(10 mice) and model group(20 mice).The mice in Group A were given subcutaneous injection of phosphate buffer while in the model group were given subcutaneous injection of bleomycin .The mice in the model group were divided into Group B(Ashcroft semi-quantitative score≥3,11 mice) and Group C(Ashcroft semi-quantitative score<3, 9 mice).The mice in Group B and Group C were injected with bleomycin to establish SSC-PF mouse model.Masson staining and HE staining were used to observe the pathological and morphological changes in lung tissues of the mice . Reverse transcription-polymerase chain reaction ( RT-PCR ) and enzyme-linked immunosorbent assay ( ELISA ) were used to test the contents of TGF-β1,TGF-β1 mRNA in the peripheral blood of lung tissues of the mice in three groups .The alkali hydrolysis was used to detect the content of hydroxyproline in lung tissues ,and Ashcroft semi-quantitative scores of lung tissues was calculated .An analysis on the correlation between the indicators was conducted .Results The Ashcroft semi-quantitative score of lung tissues,the contents of hydroxyproline,TGF-β1 in peripheral blood and TGF-β1 mRNA in lung tissues in Group B were significantly higher than those in Group A and Group C(P<0.05).But the Ashcroft semi-quantitative score of lung tissues,the contents of hydroxyproline,TGF-β1 mRNA in lung tissues and TGF-β1 in peripheral blood showed no significant difference between Group A and Group B(P>0.05).The contents of TGF-β1 mRNA in lung tissues and TGF-β1 in peripheral blood positively correlated with the Ashcroft semi-quantitative score of lung tissues as well as the content of hydroxyproline(P<0.05).Conclusion TGF-β1 is highly expressed in the model mice with SSc-PF,and increases with the increase of SSc-PF lesion severity .关键词
系统性硬化病/肺纤维化/转化生长因子-β1/羟脯氨酸/小鼠Key words
Systemic sclerosis/Pulmonary fibrosis/Transforming growth factor-β1/Hydroxyproline/Mouse分类
医药卫生引用本文复制引用
黎愉,张建全,李宇,钟小宁,雷玲,褚淑媛..转化生长因子-β1与系统性硬化病-肺纤维化相关性的实验研究[J].广西医学,2014,(4):420-423,4.基金项目
广西教育厅科研课题 （）
