磷脂酰肌醇3-激酶／蛋白激酶B信号通路对生长激素介导的心肌成纤维细胞增殖的影响OACSTPCD
Effect of Phosphatidylinositol-3-Kinase/Protein Kinase B Signaling Pathway on Growth Hormone-Induced Proliferation of Cardiac Fibroblasts
目的：探讨生长激素（ GH）对心肌成纤维细胞（ CFs）增殖的影响及其作用机制。方法以不同浓度的重组人生长激素（rhGH）（100、200、500、1000 ng/ml）和磷脂酰肌醇3-激酶/蛋白激酶B（PI3K/Akt）抑制剂LY294002（10μmol/L）单独或同时作用CFs 48 h后，采用MTT法检测细胞增殖，Wertern Blot检测PI3K/Akt信号途径中Akt和磷酸化Akt （ p-Akt ）的蛋白水平的变化。结果 rhGH…查看全部>>
Objective To investigate the effect of growth hormone ( GH) on the proliferation of cardiac fibroblasts (CFs) and its mechanism.Methods CFs were treated by different concentrations of recombinant human growth hormone (rhGH) (100,200,500,1000 ng/ml) with or without phosphatidylinositol-3-kinase/protein kinase B ( PI3K/Akt) inhibitor LY294002 (10μmol/L) for 48 hours.MTT assay was used to determine the proliferation of CFs .Western Blot was used to detect the p…查看全部>>
刘洪沛
重庆医科大学附属第一医院酉阳县人民医院心内科，酉阳县 048200
医药卫生
心肌成纤维细胞生长激素磷脂酰肌醇3-激酶/蛋白激酶B信号通路
Cardiac fibroblastsGrowth hormonePhosphatidylinositol-3-kinase/protein kinase B signaling pathway
《广西医学》 2014 (7)
873-875,3
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