酶联免疫吸附试验与流式细胞术检测血小板自身抗体的比较OACSTPCD
Enzyme-Linked Immunosorbent Assay versus Flow Cytometry in Detection of Platelet Autoantibody:A Comparative Analysis
目的:比较酶联免疫吸附试验( ELISA)与流式细胞术( FCM)检测血小板自身抗体的特点,探讨其对特发性血小板减少性紫癜( ITP)的诊断意义。方法42例ITP患者,分别采用FCM和ELISA检测血小板表面及血浆中的抗血小板自身抗体。结果 FCM检出血小板自身抗体阳性36例,阳性率为85.7%;ELISA检出血小板自身抗体阳性30例,阳性率为71.4%,两种方法阳性率差异无统计学意义( P>0.05),但是FCM和ELISA检测血小板表面抗体阳…查看全部>>
Objective To compare the charateristics of enzyme-linked immunosorbent assay ( ELISA ) and flow cytometry ( FCM ) in detection of platelet autoantibodies , and to study the diagnostic significance of idiopathic thrombocytopenic purpura ( ITP) .Methods The platelet autoantibodies in platelet memberane and seurm of 42 ITP patients were detected by ELISA and FCM .respectively .Results The result of FCM showed that 36 cases of platelet autoantibody positive ,wit…查看全部>>
周燕;钟周琳;刘金莲
广西南宁输血医学研究所,南宁市 530003广西南宁输血医学研究所,南宁市 530003广西南宁输血医学研究所,南宁市 530003
医药卫生
血小板减少性紫癜血小板自身抗体酶联免疫吸附实验流式细胞术
Idiopathic thrombocytopenic purpuraPlatelet autoantibodyEnzyme-linked immunosorbent assayFlow cytometry
《广西医学》 2014 (8)
1037-1039,3
广西科学研究与技术开发计划项目(08160-05)
评论