广西医学Issue(10)：1357-1359,3.DOI:10.11675/j.issn.0253-4304.2014.10.01
细胞相互作用和阿托伐他汀对内皮细胞与系膜细胞 PAF 及其受体基因表达的影响
Effect of Atorvastat in on th e Ro le of Platelet Activat ing Factor and Expre ssion Level of Platelet Activating Factor Receptor in Mesangial Cells via Intera ction of Endothelial Cells and Mesangial Cells
摘要
Abstract
Objective To investigate the role of platelet activating factor (PAF) and the expression of platelet activating factor receptor (PAF-R) in mesangial cells affected by the interaction of endothelial cells and mesangial cells exposed to high glucose and lysophosphatidylcholine (LPC),and to explore the effect of atorvastatin intervention . Methods Endothelial cells and mesangial cells were co -cultured while mesangial cells were cultured alone .The model of intercellular interaction between endothelial cells and mesangial cells was established and divided into 4 groups:control group,mannitol group ,high glucose and LPC group,and atorvastatin group.The expression of PAF-R mRNA in mesangial cells was determined by quantitative real -time PCR.The content of PAF in mesangial cells was determined by enzyme-linked immunosorbent assay( ELISA).Results The content of PAF and the expression level of PAF-R in mesangial cells increased when exposed to high glucose and LPC in co-culture and single-culture groups(P <0.05).The content of PAF of the monolayer cell culture was lower than that of the co-culture(P <0.05).Atorvastatin could inhibit the up-regulation of PAF and PAF-R mRNA expression in mesangial cells induced by high glucose and LPC(P <0.05).Conclusion Exposed to high glucose and LPC,endothelial cells and mesangial cells can interact with each other to produce PAF.The expression of PAF-R in mesangial cells is promoted by high glucose and LPC.The intercellular interaction between endothelial cells and mesangial cells may be intervened by atorvastatin,atorvastatin can eliminate the excess of the PAF and down -regulate the expression of PAF-R in mesangial cells when exposed to high glucose and LPC .关键词
系膜细胞/内皮细胞/阿托伐他汀/血小板活化因子/血小板活化因子受体Key words
Mesangial cell/Endothelial cell/Atorvastatin/Platelet activating factor/Platelet activating factor receptor分类
医药卫生引用本文复制引用
周素娴,李浩,邓婷婷..细胞相互作用和阿托伐他汀对内皮细胞与系膜细胞 PAF 及其受体基因表达的影响[J].广西医学,2014,(10):1357-1359,3.基金项目
国家自然科学基金资助项目（81260134）；广西自然科学基金资助项目（2011GXNSFB018108）；广西中医药管理局科研项目 （）
