广西医学Issue(11)：1526-1528,1537,4.DOI:10.11675/j.issn.0253-4304.2014.11.06
SUMO 特异性蛋白酶1在心肌组织缺血／再灌注损伤中的保护作用
Protective Effect of SUMO-Specific Protease 1 on Ischemia/Reperfusion Injury of Myocardial Tissue
顾剑民 1薛松1
作者信息
- 1. 上海交通大学医学院附属仁济医院心血管外科，上海市 200127
- 折叠
摘要
Abstract
Objective To investigate the protective effect of SUMO -specific protease 1 ( SUMO-SENP1 ) on ischemia/reperfusion injury of myocardial tissue .Methods Fourteen cases of right atrial tissue before and after cardiopulmonary bypass heart operation were selected , the mouse model of ischemia reperfusion was established , and real-time quantitative polymerase chain reaction ( PCR) was used to detect the changes of SENP1 mRNA in myocardial tissue of patients and rats.The LDH release rate was detected after hypoxia reoxygenation on H 9C2 cell line,and the cell death was observed .Results The results of real-time PCR showed that the SENP1 mRNA content in myocardial tissue of14 cases after reperfusion was(4.845 ±1.248),which was significantly higher than that before reperfusion (P<0.01).The SENP1 mRNA content in myocardium of ischemia treatment rats was slightly higher than that of normal rats , and increased with the increasing reperfusion time .The mRNA level of SENP1 in H9C2 cells of specific siRNA knockout rats decreased to 30%of the controls 48 hours later,and LDH release in H9C2 cells of knockout SENP1 increased after hypoxia reoxygenation .Conclusion SENP1 has protective effects on myocardial ischemia reperfusion .The lack of SENP1 might aggravate the myocardial injury .关键词
心肌缺血/SUMO特异性蛋白酶1/心肌缺血再灌注损伤/低氧诱导因子Key words
Myocardial ischemia/SUMO-specific protease 1/Ischemia/reperfusion injury/Hypoxia-inducible factor分类
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顾剑民,薛松..SUMO 特异性蛋白酶1在心肌组织缺血／再灌注损伤中的保护作用[J].广西医学,2014,(11):1526-1528,1537,4.
