广西医学Issue(2)：137-139,3.DOI:10.11675/j.issn.0253-4304.2015.02.01
多西环素抑制碱烧伤大鼠角膜新生血管形成
Inhibitory effect of doxycycline on neovascularization in a rat model of corneal alkali burn
摘要
Abstract
Objective To investigate the inhibited effect of doxycycline on neovascularization ( NV) in a rat model of corneal alkali burn and its mechanism.Methods Thirty-two healthy SD rats were randomly divided into control group and doxycycline-treated group,with 16 rats in each group.After the corneal alkali burn model was established ,the doxycycline-treated group was given 0.5% doxycycline eye drop,while the control group was given the solution of eye drop without doxycycline .The inflammatory index was observed 3,7,14 and 21 days after injury ,respectively ,and the area of corneal NV was calculated .The cornea was detected for the expression of vascular endothelial growth factor(VEGF) by enzyme-linked immunosorbent assay(ELISA).Result s The doxycycline-treated group showed slighter conjunctival congestion and corneal edema at all time points compared with the control group .The inflammatory indexes 3,7,14 days after injury in the doxycycline-treated group were significantly lower than those in the control group (P<0.05).The areas of corneal NV 3,7,14,21 days after injury in the doxycycline-reated group were smaller than those in the control group (P<0.05).There was no significant difference in the expression of corneal VEGF between two groups (P>0.05).Conclusion Doxycycline can relieve the corneal inflammatory reaction and corneal neovascularization in rats with corneal alkali burn ,which might be associated with the inhibition of inflammatory reaction ,but might not be associated with the expression of VEGF .关键词
多西环素/碱烧伤/角膜/新生血管/大鼠Key words
Doxycycline/Alkali burn/Cornea/Neovascularization/Rat分类
医药卫生引用本文复制引用
邹文进,刘祖国,黄海,魏茜敏..多西环素抑制碱烧伤大鼠角膜新生血管形成[J].广西医学,2015,(2):137-139,3.基金项目
国家自然科学基金（81160119）；广西自然科学基金（2012 GXNSFAA053107）；广西高等学校立项科研项目（201204LX064）；广西医药卫生科研课题（Z2009107），广西医科大学博士启动基金 （）
