广西植物Issue(3)：299-303,5.DOI:10.3969/j.issn.1000-3142.2014.03.004
应用 matK 基因鉴别青天葵及其常见混伪品
Apply matK gene to identify Nervilia fordii and its common adulterants
摘要
Abstract
In order to establish the novel method on distinguishing Nervilia fordii and its common adulterants,matK genes from N .fordii and its adulterants were amplified and sequenced using a pair of universal primes.The results revealed that matK genes acquired from N .fordii and its adulterants contained 587 base pairs,and average GC con-tent was 32.8%.The intraspecific distances among N .fordii were 0,whereas the interspecific distance between N . fordii and its adulterants ranged from 0.016 to 0.375.The clustering tree according to Neighbor Joining method and Kimura 2-Parameter model discriminated N .fordii and its adulterants obviously.matK gene is suggested to be an effective biomarker to identify N .fordii and its common adulterants.关键词
青天葵/matK 基因/混伪品Key words
Nervilia fordii/matK gene/adulterant分类
农业科技引用本文复制引用
黄琼林,梁凌玲,何瑞,詹若挺,陈蔚文..应用 matK 基因鉴别青天葵及其常见混伪品[J].广西植物,2014,(3):299-303,5.基金项目
教育部博士点基金(200805720004) （200805720004）
教育部留学回国人员科研项目(教外司留[2009]1001) （教外司留[2009]1001）
