广西植物Issue(4)：436-441,6.DOI:10.3969/j.issn.1000-3142.2014.04.002
广西五种绞股蓝属植物离体快繁与种质保存研究
Study on rapid propagation and germplasm conservation in vitro of five species of genus Gynostemma in Guangxi
摘要
Abstract
Using stem with buds as explants,experiments were conducted to observe the effects of different concentra-tion 6-BA and NAA 0.02 mgL-1 on induction,differentiation,proliferation of Gynostemma in Guangxi,in order to establish their rapid propagation systems.Meanwhile,different concentrations of the mineral nutrition,sucrose and plant grow inhibitors (CCC,ABA,PP333 )were researched.The results showed that the best induction medium was MS medium supplemented with 2.0 mgL-1 6-BA and 0.02 mgL-1 NAA.The suitable proliferation medium for G . compressum was MS+6-BA 2.0 mgL-1 +NAA 0.02 mgL-1 and MS medium supplemented with 1.5 mgL-1 6-BA and 0.2 mgL-1 NAA was best for others.The rooting medium was 1/2MS medium with 0.02 mgL-1 NAA, accounting for 100% of rooting rate.1/2MS medium and sucrose 40 gL-1 were the most suitable for the conserva-tion in vitro of all species.Plant grow inhibitors could slow down the grow speed effectively,and the best kind and concentration were ABA 1.0 mgL-1 and CCC 1.0 mgL-1 .Several species were inhibited by CCC 1.0 mgL-1 and the survival rate was up to 94.5% after being conserved 360 d.PP333 was not suitable for all species to conserve in vitro .Vigor testing showed that the ability of propagating and rooting of the plantlets didn’t fall down.关键词
绞股蓝/扁果绞股蓝/广西绞股蓝/光叶绞股蓝/长梗绞股蓝/组织培养/离体保存Key words
Gynostemma pentaphyllum/G .guangxiense/G .compressum/G .laxum/G .longipes/tissues culture/conservation in vitro分类
农业科技引用本文复制引用
姚绍嫦,谢月英,黄雪彦,余丽莹..广西五种绞股蓝属植物离体快繁与种质保存研究[J].广西植物,2014,(4):436-441,6.基金项目
广西科技攻关项目(桂科攻11107010-3-3) （桂科攻11107010-3-3）
