广西植物Issue(4)：552-556,5.DOI:10.3969/j.issn.1000-3142.2014.04.024
DNA 条形码技术在毒品原植物大麻鉴定中的应用
Application of DNA barcoding in cannabic identification
摘要
Abstract
To study the feasibility of DNA barcoding on cannabic species identification,DNA were extracted from sixty cannabis plants’leaves(cultivated cannabis from Inner Mongolia,Heilongjiang,Shanxi Yan’an and Yulin,wild canna-bis from Xinjiang Manas,six male and female cannabis from each region),used ribosomal DNA intergenic region ITS2 universal primers and cpDNA psbA-trnH primer for PCR amplification,then sequenced in both directions,the results compared to manual correction and finally for Blast comparison.ITS2’amplified sequences of all samples were completely consistent,but psbA-trnH’amplified sequences varied greatly,which were detected eight kinds of cpDNA haplotypes.MEGE 5.1 software was used to calculate the genetic distance between species,and build NJ phylogenetic trees which could effectively separate the five regions of cannabic samples.So DNA barcoding on cannabic species identification was proved to have viability,but the accuracy,reliability and possibilities of identification of its origin and species need further research.关键词
DNA 条形码/大麻/种属鉴定Key words
DNA barcoding/cannabis/species identification引用本文复制引用
宋炳轲,杨雪莹,倪萍娅,裴黎,张颖,徐小玉..DNA 条形码技术在毒品原植物大麻鉴定中的应用[J].广西植物,2014,(4):552-556,5.基金项目
公安部物证鉴定中心基本科研业务费专项(2010JB002) （2010JB002）
