广西植物Issue(5)：714-718,5.DOI:10.3969/j.issn.1000-3142.2014.05.024
四川宜宾七种中药提取物体外抗菌活性研究
Antimicrobial activity in vitro of crude extracts from seven kinds of traditional Chinese medicine from Yibin in Sichuan
摘要
Abstract
The antimicrobial activities of crude extracts from seven kinds of traditional Chinese medicine from Yibin in Sichuan against E.coli,Salmonella and Pasteurella in vitro were investigated with serial two-fold broth dilution method and agar plate counting method.The results showed that the crude extract from Phello-dendron amurense had the strongest antimicrobial activity,its Minimum inhibitory concentration(MIC)range was 15.6-125 mg·mL-1,its minimum bactericidal concentration(MBC)was 31.25-250 mg·mL-1.The anti-microbial activity of crude extract from Gardenia jasminoides was worse than Phellodendron amurense,its MIC was 62.5-250 mg·mL-1 ,and MBC was 125->250 mg·mL-1 .The crude extract from Citrus medica var.sarcodactylis had stable antimicrobial activity,its MIC all was 125 mg·mL-1 ,MBC was 125 mg·mL-1 and 250 mg·mL-1.The other four extracts from Eucommiaulmoides,Fallopiamultiflora、Curcumalonga, Lonicera similis were proved that their antimicrobial activities were not as strong as other three extracts. Most of their MIC were≥250 mg·mL-1 .The results indicated that the extracts of Phellodendron amurense and Gadenia jasminoides had better bacteriostasis effect,and extract of Citrus medica var.Sarcodactylis was stable.They might be potential agents against bacterial disease,so they are worth of further study.关键词
四川宜宾/中药提取物/抗菌活性/MIC/MBCKey words
Yibin in Sichuan/extracts from traditional Chinese medicine/antimicrobial activity/MIC/MBC分类
农业科技引用本文复制引用
李莉,殷中琼,贾仁勇,代如意,李曼,蔡红..四川宜宾七种中药提取物体外抗菌活性研究[J].广西植物,2014,(5):714-718,5.基金项目
国家“十二五”科技支撑计划项目(2011BAD34B03) （2011BAD34B03）
四川省青年科技创新研究团队(2013TD0015) （2013TD0015）
