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广西毛竹种质资源 AFLP 分析

杨春生 卢永彬 林燕芳 唐绍清 周海平 李晓铁

广西植物Issue(6):742-746,787,6.
广西植物Issue(6):742-746,787,6.DOI:10.3969/j.issn.1000-3142.2014.06.003

广西毛竹种质资源 AFLP 分析

AFLP analysis of genetic relationships among Phyllostachys edulis germplasm resource in Guangxi

杨春生 1卢永彬 2林燕芳 2唐绍清 2周海平 3李晓铁3

作者信息

  • 1. 广西桂林市森林病虫害防治检疫站,广西 桂林 541001
  • 2. 广西师范大学生命科学学院、珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西 桂林 541004
  • 3. 广西桂林市林业科学研究所,广西 桂林 541004
  • 折叠

摘要

Abstract

Thirty-three Phyllostachys edulis germplasms from 11 provenances in Guangxi were analyzed by AFLP markers.Five pairs of primer combination were used to amplify the genomic DNA.A total of 198 AFLP bands were obtained,47 (23.74%)of them were polymorphic.The genetic distance between the 11 provenance ranged from 0.000 6 to 0.136 9.The tested gemplasms can be divided into 4 categories by UPGMA clustering analysis.Nandan and Zhaoping provenances were clustered into two categories respectively.They were distinct to other two categories. PCA analysis results were consistent with the results of cluster analysis.Mantel test results showed that the correla-tions between genetic distance and geographic distance in P .edulis provenance sources were significant (r =0.555 8, P =0.555 8),and AFLP was an effective marker to identify germplasm of P .edulis .

关键词

毛竹/种质资源/遗传关系/AFLP

Key words

Phyllostachys edulis/germplasm resource/genetic relationship/AFLP

分类

农业科技

引用本文复制引用

杨春生,卢永彬,林燕芳,唐绍清,周海平,李晓铁..广西毛竹种质资源 AFLP 分析[J].广西植物,2014,(6):742-746,787,6.

基金项目

广西区林业重大项目(桂林科字[2010]第8号 ()

桂林科字[2009]第18号) ()

广西科技攻关项目(桂科攻1123004-5) (桂科攻1123004-5)

桂林市科技攻关项目(20100509) (20100509)

广西植物

OA北大核心CSCDCSTPCD

1000-3142

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