广西植物Issue(1)：31-35,5.DOI:10.11931/guihaia.gxzw201309021
厚壁毛竹与毛竹叶表皮微形态特征比较研究
Micromorphological study on leaf epidermis of Phyllostachys edulis‘Pachyloen’and Phyllostachys edulis
摘要
Abstract
Micromorphological characteristics of leaf epidermis of Phyllostachys edulis‘Pachyloen’and Phyllostachys edulis were observed by scanning electron microscope.The results indicated that the micromorphological characteris-tics of leaf epidermis of two bamboo species were similar with relatively simple adaxial epidermis only composed of long cell,short cell and bulliform cell.While the abaxial epidermises were complicated,which had papilla,microhair, prickle,silica and stomata.There were three papilla forms:round,granular and rod papillae.Round papilla was the largest distributing in longitudinal rows.The granular papillae were the smallest widely distributing in the abaxial ep-idermises.And the rod papillae were located surrounding the stomata.The microhairs were sparse and composed of two cells with the apical cell withered,possibly because of the thin cell walls and the top cell,whose cell wall was thick.The stomata were composed of subsidiary cells and kidney-shaped guard cells,and distributed among long cells between the veins of the abaxial epidermises with four papillae overarhing them.Bristles with sharp tops were widely distributed in the abaxial epidermises and the cell walls were thin.The bristles were located parallelly with the epider- mis and surrounded by four granular papillae.Silicon cells and cork cells were often located in pairs among the long cells in the epidermis.And they were slightly sunken,round or nearly round.No silicon cell and cork cell distributed between veins.The remarkable differences between leaf epidermis of Phyllostachys edulis ‘Pachyloen’and Phyl-lostachys edulis were the length and density of stomata on the abaxial epidermis:the stomata of P.edulis ‘Pachy-loen’were longer than that of P.edulis except those of two-year-old ones,while its stomatal density was low.There was no significant difference of papilla density between both two bamboo species and different ages of the same spe-cies.While stomatal density and axial length change strikingly with aging.The stomatal density of one-year-old P. edulis ‘Pachyloen’was 609±25 per mm2 ,larger than those of the two-year-old and five-year-old bamboo.The axial length of the stomata of P.edulis ‘Pachyloen’changed as “V”with aging,that was the axial length of the stomata of two-year-old P.edulis ‘Pachyloen’was the smallest,(18.909±1.322)μm.The same rule was in the changes of stomatal density of P.edulis,while the largest was that of the five-year-old bamboo,and the stomatal axial length of P.edulis leaves become longer with aging.The results did accord with the development of the culm.The results of analysis of variance about the micromorphological characteristics of leaf epidermis of two bamboo species showed that there was no dramatic difference of papilla density,stomatal density and axial length of the stomata in the abaxial epi-dermises between the same ages of both two bamboo species (P>0.05).The similar characteristics of leaf epidermis also showed that the systematic relationship of two bamboo species.This study is important to elucidate the structural foundation of forming thicker culm,to make an intensive study and good use of the new bamboo variety,and will pro-vide theoretical basis for raising the bamboo biomass.关键词
厚壁毛竹/毛竹/营养叶/表皮Key words
Phyllostachys edulis ‘Pachyloen’/Phyllostachys edulis/trophophyll/epidermis分类
农业科技引用本文复制引用
刘腾飞,杨光耀,于芬..厚壁毛竹与毛竹叶表皮微形态特征比较研究[J].广西植物,2015,(1):31-35,5.基金项目
国家自然科学基金(31000289,30760204) （31000289,30760204）
江西省自然科学基金(2009GQN0011) （2009GQN0011）
