广西植物Issue(1)：120-125,6.DOI:10.11931/guihaia.gxzw201312021
油茶茶枯中茶皂素的提取工艺研究
Extraction of tea saponin from Camellia cake
摘要
Abstract
Camellia cake is a common by-product of processing,which contains high content of tea saponin.Tea sapo-nin is widely used in industrial production as a surfactant,which has excellent performance.But the method of extrac-tion technology is not mature currently.One new method was proposed in the article,which used defatted Camellia cake powder as raw material,ethanol concentration as extraction agent,the extraction rate and content of tea saponin as extraction effect evaluation index.In order to acquare the extraction rate and content of tea saponin from Camellia cake,six factors such as the influences of the extraction time,adding quantity of 3%NaOH,ethanol concentration,the ratio of solid-liquid,temperature and times of extraction were studied.After single factor experiment and orthogonal experiment,the optimized conditions for extracting tea saponin were established by orthogonal experiments,which in-cluded that the ratio of solid to liquid was 1∶17,ethanol concentration was 75%,extracting temperature was 81 ℃, extraction time was 88 min,adding quantity of 3% NaOH was 2.5 mL and extraction times were 2 etc.Under these conditions,the content of tea saponin from Camellia cake was 0.53 mg·mL-1 and the yield of extraction reached 21.35%.After adding the NaOH ,the yield of extraction improved significantly under the optimum conditions.The experimental method has good reference value for optimizing the extraction of tea saponin in the future.关键词
油茶茶枯/茶皂素/提取Key words
Camellia cake/tea saponin/extraction分类
农业科技引用本文复制引用
龚秋实,肖瑜,梁美娜,刘以清,黄浦..油茶茶枯中茶皂素的提取工艺研究[J].广西植物,2015,(1):120-125,6.基金项目
国家自然科学基金(21367010) （21367010）
