广西植物Issue(2)：156-160,5.DOI:10.11931/guihaia.gxzw201403040
十字花科多肽激素类基因 RALF 的同源基因克隆及进化分析
Cloning and evolutionary analysis of homologous sequences of RALF in Cruciferae
摘要
Abstract
Peptide hormones genes are important in plant growth and development and RALF (rapid alkalinization factor)is one of them.Cruciferae is a big family in Chinese vegetable crops.Polypeptide hormone gene RALFbn was cloned from Brassica campestris (rape)in the authors’previous work.In order to find out the information of RALF homologous genes in Cruciferae vegetable crops,this experiment was done.At this time,the primers were designed according to the sequence of RALFbn gene.The genomic DNAs of 7 important vegetable crops were extracted in ad-vance.The vegetable materials were selected from Brassica,Raphanus,Rorippa and Barbarea and they all belonged to the same family of Cruciferae.Seven sequences homologous to RALF were cloned from the 7 materials from impor-tant vegetable crops with the primers designed from RALFbn .Then the 7 DNA sequences together with the RALFbn gene were analyzed.The results showed that all the DNA sequences’open reading frames were about 300 bp long.There was no intron in the 7 genes.The 7 proteins encoded by them were all made by 73 amino acids.All these indi-cated that the RALF homologous genes were very conservative in the 4 genera of cruciferous species.At the same time,the 7 RALF homologous genes expression analyses were done in four genera in Cruciferae.The result indicated that these genes were not or weakly expressed in root,stem,leaf,inflorescence axis and other vegetative organs,while malnly expressed in reproductive organs,in which,the total amounts of the expression in flower buds and open flow-ers were generally higher than those in tender siliques.All the expression indicated that the physiological active period of these genes in Cruciferae was in the flower growth period.The system tree was constructed with the 7 RALF ho-mologous genes in the 4 genera of Cruciferae.The NJ tree of RALF homologous genes reflected that RALFbn ,Bo-RALFj x and BoRALFzh formed a branch while BjRALFdn4 and BjRALFxlh formed another branch with the rest RALF homologous genes from Raphanus,Rorippa,Barbarea clustered in the same branch.The evolutionary pathway of these RALF homologous genes in a certaln extent also reflected the genetic background relationships between these crops.In all,this study enriched the information of the rapid alkalinization factor (RALF )family and expanded the knowledge of the molecular evolution data of Cruciferae.关键词
十字花科/多肽激素类基因/RALF/同源基因/进化分析Key words
Cruciferae/polypeptide hormone gene/RALF/homologous gene/evolutionary analysis分类
农业科技引用本文复制引用
聂传朋,李尚擘,刘瑞姣,李焰焰..十字花科多肽激素类基因 RALF 的同源基因克隆及进化分析[J].广西植物,2015,(2):156-160,5.基金项目
国家自然科学基金(31000132) （31000132）
国家大学生创新创业训练计划项目(201310371015) （201310371015）
