广西植物Issue(2)：250-254,238,6.DOI:10.11931/guihaia.gxzw201307019
二花蝴蝶草的组织培养及植株再生
Tissue culture and plant regeneration from Torenia biniflora
摘要
Abstract
Torenia biniflora is a member of Scrophulariaceae Torenia ,and it is an annual flowering plant that widely grows in Chinese subtropical area,such as Guangdong Province and Guangxi Zhuang Autonomous Region.T .bini-flora is a wild flower with high great ornamental value.However,in natural growth condition,the reproduction speed of T .Biniflora is slow,proliferation rate is low,and the kinds of flower colour and type are few,therefore,it is una-ble to meet the diversified need of market.Plant tissue culture technology provides a new way to improve and breed new varieties for ornamental plants.Up to now,a lot of members of Torenia plants have been successfully established in vitro regeration system through tissue culture,such as T .fournieri Linden,T .balllonii Susie Wong and T .con-color Lindl.,etc.While,tissue culture for T .Biniflora has never been reported.The maln objective of this research was to establish in vitro regeneration system of T .Biniflora through tissue culture and study the effects of plant growth substances such as IBA,6-BA and NAA for clonal multiplication.In this study,we created a reliable protocol for regenerating T .biniflora plants in tissue culture by using leaf explants.The effects of different concentrations and combinations of plant growth substances on adventitious bud induction and growth were studied,and the effects of different ionic strengths of media and concentrations of IBA on rooting were analyzed.According to a series of indi-ces,such as average number of buds,frequency of bud induction,frequency of rooting,average number of root and av-erage length of root,the optimal media for inducing adventitious buds and rooting were screened respectively.The re-sults showed that adventitious bud induction was related with the concentrations and combinations of plant growth regulator,and the optimal medium for buds induction was MS+6-BA 0.5 mg•L-1 +NAA 0.2 mg•L-1 .The best medium for rooting was 1/2MS as basal medium with IBA 0.05 mg•L-1 .In this study the in vitro plant reproductive system of T .biniflora with high frequence was completely established,and it would provide an important experiment foundation for the research of rapid propagation and genetic transformation for T .biniflora .关键词
二花蝴蝶草/不定芽/不定根/再生植株Key words
Torenia biniflora/adventitious buds/adventitious root/regenerated plant分类
农业科技引用本文复制引用
王瑛华,石秋英,陈雄伟,陈刚,金红..二花蝴蝶草的组织培养及植株再生[J].广西植物,2015,(2):250-254,238,6.基金项目
肇庆市科技项目(2008N001) （2008N001）
肇庆学院自然科学基金(0731) （0731）
