广西植物Issue(3)：414-419,407,7.DOI:10.11931/guihaia.gxzw201311045
24份甜樱桃材料自交不亲和S基因型鉴定
Identification of self-incompatibility genotypes of 24 sweet cherries
摘要
Abstract
Sweet cherry exhibits the monofactorial gametophytic self-incocompatibility system,the correct evaluation and rational utilization on sweet cherry cultivars have been limited because of the self-incompatibility,therefore,determina-tion of correct pollen incompatibility groups and assignment of cultivars to the groups are essential for good crop produc-tion.With 24 sweet cherry cultivars as materials,using 5 pairs of prunus primer combinations to amplify S-allele-specific PCR for 24 kinds of sweet cherries and S-genotypes of sweet cherry cultivars were identified,the S-gene amplified frag-ments were cloned and searched in GenBank,and combined with 5 primer combinations,S-genotype of 24 cultivars were identified.The conclusion was as follows:the same S genes displayed the same size fragments in electrophoresis;The si-zes of S-gene amplification fragments were S1 800 bp,S3 762 bp,S4 962 bp,S5 300 bp,S6 456 bp,S9 650 bp.And the S genotypes of the tested self-incompatible cultivars were identified that Hongshouqiu and Early Ruby were S1S3;Lapins was S1S4;Ruby was S1S6;Brooks was S1S9;Napoleon was S3S4;Qinlin,Tartarian,Van,Zaodaguo,Lizhu,Meizao, 5-106 and Satonishiki the same as Santina were S3S6;Heizhenzhu,Hongdeng,Samituo and Qinying were S3S9;Victor was S5S9;Mingzhu,Hongmi and Rainier the same as Bing were S6S9.关键词
甜樱桃/自交不亲和/S基因型Key words
sweet cherry/self-incompatibility/S-genotypes分类
农业科技引用本文复制引用
陈仲刚,吕秀兰,王进,阳姝婷,涂勋良,赖静..24份甜樱桃材料自交不亲和S基因型鉴定[J].广西植物,2015,(3):414-419,407,7.基金项目
四川省科技支撑计划项目(2013NZ0008) （2013NZ0008）
