广西植物Issue(3)：420-427,8.DOI:10.11931/guihaia.gxzw201403048
番茄 RGA4蛋白与马铃薯 Rpi-blb1蛋白的同源比较分析
Homology analysis of tomato RGA4 and potato Rpi-blb1 protein
摘要
Abstract
Using electronic cloning method,the putative disease resistance protein RGA4 and the homologue of the potato Rpi-blb1protein were obtained from tomato’s protein sequences database in NCBI by protein-blast when the sequence of late blight resistant protein Rpi-blb1 from potato was used as query sequence,then,they were compared in gene mapping,gene structure,modify,secondary structure,topological structure and electronic gene expression pro-file,etc,for proving their evolutionary relationship.The result showed that tomato RGA4 protein (XP_004245923.1) was composed of 988 amino acid residues with NB-ARC and LRR8 conserved domain,the corresponding cDNA se-quence (XM-004245875.1)was located on SL2.40 region in the chromosome 8,with genomic DNA length 4 089 bp, the alignment region in chromosome 8 between 57228847 - 57232935 bp,and composed of two exons and one intron.The tomato RGA4 protein was an unstable globular secretory protein without transmembrane structure.To-mato RGA4 protein was regarded as an orthologous of potato Rpi-blb1 protein because they were similar in secondary structure composition,gene sequence composition,gene mapping,etc,but their minor differences in gene evolution and composition could result in different functions.This study would provide theory for cloning late blight resistant gene and its utilization in tomato breeding of disease resistance.关键词
番茄/晚疫病/垂直同源/生物信息学分析Key words
Solanum lycopersicum/late blight/orthologous/bioinformation analysis分类
农业科技引用本文复制引用
武安泉,王俊生,阮先乐,范小芳,陈龙..番茄 RGA4蛋白与马铃薯 Rpi-blb1蛋白的同源比较分析[J].广西植物,2015,(3):420-427,8.基金项目
河南省教育厅基金(14B210022) （14B210022）
河南省高校博士科研启动经费(ZKSYBSCX201110) （ZKSYBSCX201110）
