工业技术创新Issue(4)：432-435,4.DOI:10.14103/j.issn.2095-8412.2014.04.009
脂肪酶固定化及酶解制备甘油二酯研究
Research on Lipase Immobilization and Preparation of Diacylglycerol
摘要
Abstract
The immobilization of lipase and enzyme hydrolyzation technology were studied for preparing of the diglyceride in this paper. Lipase (L04) was immobilized by 12 kinds of resins, the excellent resin was selected as subject to optimize further for preparing diglyceride. The results shown that the resin modified, the lipase (L04) was immobilized onto the resin containing tertiary amino groups (ESQ-1-X) and the mass ratio of ESQ-1-X to lipase (L04) was found to be 15:1, possessed excellent lipase activity. 56.1% of the diglyceride in the reaction system was acquired by adding 0.1g/g (lipase/resin) of the resin modified and the reaction time was 20h, the yield of diglyceride remained fairly constant after having recycled for 6 times.关键词
甘油二酯/脂肪酶/固定化酶Key words
Diacylglycerol/Lipase/Immobilization of lipase分类
轻工纺织引用本文复制引用
杨海燕,吴啟旋,李波,贾佳..脂肪酶固定化及酶解制备甘油二酯研究[J].工业技术创新,2014,(4):432-435,4.基金项目
深圳市战略新兴产业发展专项项目(CXZZ20120619140117384) （CXZZ20120619140117384）
