工业微生物Issue(1)：20-24,5.DOI:10.3969/j.issn.1001-6678.2015.01.004
一株孕酮转化工程菌的构建
Construction of genetically engineered strain for bioconversion of progesterone
刘园园 1魏维 2戈梅 3陈代杰1
作者信息
- 1. 上海师范大学，上海200234
- 2. 上海交通大学，上海200240
- 3. 上海来益生物药物研发中心，上海201203
- 折叠
摘要
Abstract
Progesterone is not only a commonly used drug in clinic,but also a kind of important medicine intermediate in cortisol or cortisone synthesis. Thus a convenient method to produce progesterone is important in steroid drug production. In this study,a recombinant plasmid BL21/pET28b-choE was constructed by cloning choE gene from Rhodococcus erythropolis CGMCC 1. 2362 to express cholesterol oxidase in E. coli. Results showed that this recombinant strain could transform pregnenolone to progesterone. When the concentration of pregnenolone was up to 0. 2 g/L,the bioconversion efficiency was more than 80%.关键词
生物转化/孕酮/胆固醇氧化酶/原核表达Key words
biotransformation/progesterone/cholesterol oxidase/prokaryotic expression引用本文复制引用
刘园园,魏维,戈梅,陈代杰..一株孕酮转化工程菌的构建[J].工业微生物,2015,(1):20-24,5.
