工业微生物Issue(1)：43-49,7.DOI:10.3969/j.issn.1001-6678.2015.01.008
一株产木聚糖酶菌株的筛选鉴定及产酶条件初步优化
Screening and identification of xylanase producing strains and condition optimization for xylanase production
罗玲 1蔡俊 1王常高 1杜馨 1林建国1
作者信息
- 1. 湖北工业大学发酵工程教育部重点实验室和工业发酵湖北省协同创新中心，湖北武汉430068
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摘要
Abstract
A strain with producing xylanase ability was isolated from paper mill sludge and sewage. It was identified as Streptomyces althioticus(patent accession:No. CCTCC M 2014110). Single factor experiments such as carbon,nitrogen, temperature,initial pH,fermentation period,rotation speed,inoculum size,liquid volume were preliminarily optimized. The results showed that the optimum conditions for xylanase production were as follows:corn cob 30 g/L for the carbon source,soybean powder 20 g/L and potassium nitrate 10 g/L for nitrogen compound,fermentation period 120 h,fermenta-tion temperature 40 ℃,inoculum size 6%,initial pH 5 . 0 ,liquid volume 50 mL/250 mL flask and shaker speed 160 r/min,respectively.关键词
木聚糖酶/筛选/异硫链霉菌/产酶条件Key words
cotton stalk/protein/fermentation/preliminary optimization引用本文复制引用
罗玲,蔡俊,王常高,杜馨,林建国..一株产木聚糖酶菌株的筛选鉴定及产酶条件初步优化[J].工业微生物,2015,(1):43-49,7.
