工业微生物Issue(2)：13-20,8.DOI:10.3969/j.issn.1001-6678.2015.02.003
产普鲁兰酶重组大肠杆菌质粒稳定性的研究
Study on plasmid stability of recombinant Escherichia coli producing pullulanase
摘要
Abstract
It was found that different host bacteria would seriously affect the plasmid stability and pullulanase activity by investigating the recombinant E. coli BL21 (DE3)/pET28a-s-pul producing pullulanase. In this study,a recombinant E. coli BL21(DE3)pLysS/pET28a-s-pul was constructed with a new strain named E. coli BL21(DE3)pLysS,which suc-cessfully controlled the basal expression and improved the plasmid stability. After optimizing the medium and fermentation conditions,the pullulanase activity was enhanced from 480 U/mL to 627 U/mL with an increase of 30. 6%. The results of this research illustrated that strictly controlling the basal expression of the foreign protein was one of the important methods to improve the stability of recombinant bacteria.关键词
普鲁兰酶/大肠杆菌/质粒稳定性/本底表达/发酵优化Key words
pullulanase/E. coli/plasmid stability/basal expression/fermentation optimization引用本文复制引用
翟成一,徐岩,聂尧,穆晓清..产普鲁兰酶重组大肠杆菌质粒稳定性的研究[J].工业微生物,2015,(2):13-20,8.基金项目
国家高技术研究发展计划（863计划），食品酶及应用（2012AA022207），江苏高校优势学科建设工程资助项目。 （）
