海南师范大学学报（自然科学版）Issue(4)：412-415,4.
改良CTA B法对提取玫瑰DNA 质量的影响
Effect of the Modified CTAB Method on DNA Quantity From Rosa Rugosa
摘要
Abstract
Rosa rugosa is the plant with high phenolic substance and pigment. In order to extract the high quality DNA suitable for SRAP analysis, the modified method was used with 5 kinds of combinations of different concentration of β-mer⁃captoethanol and PVP and 2%or 3%CTAB. The results showed that it can get the high purity rose DNA from the extracts of the combination of 3% CTAB+2% β-mercaptoethanol+10% PVP. Furthermore, the electrophoresis bands of DNA were clear and exhibited no significant tailing phenomenon. The average yield of rose DNA can meet the quality and purity in SRAP technical and DNA fingerprint requirements.关键词
玫瑰/DNA/β-巯基乙醇/PVPKey words
Rosa rugosa/DNA/β-mercaptoethanol/PVP分类
生物科学引用本文复制引用
严廷良,许惠秋,张颖,修效中,苗启晨,李蕾..改良CTA B法对提取玫瑰DNA 质量的影响[J].海南师范大学学报（自然科学版）,2014,(4):412-415,4.基金项目
三亚市专项科研试制项目（2012KS09）；大学生国家级创业训练项目 （）
