解放军医药杂志Issue(5)：10-13,4.DOI:10.3969/j.issn.2095-140X.2014.05.003
大鼠骨髓基质干细胞体外分离、纯化、培养的实验研究
A Study on the Methods of Separation, Depuration and Cultivation in Vitro for Bone Marrow Stromal Cells of Rats
摘要
Abstract
Objective To explore the methods of separation, depuration and cultivation in vitro for bone marrow stromal cells ( BMSCs) of rats. Methods Five-week-old Wistar rats were anesthetized through abdominal cavities using 1% sodium pentobarbital (0. 006 ml/g), and then BMSCs were extracted from the rats'femurs and tibias. The adherent culture method was used to separate, purify and culture the BMSCs. The changes in cell morphology were observed, and characteristics of cell growth, passage and amplification were studied by drawing curve of growth. The cells were identi-fied with flow cytometry. Results The BMSCs obtained with adherent culture method had rapid proliferation and good growth curve, which proved BMSCs by the detection of cell surface marker, and the cells had good homogeneity (96. 36%). Conclusion Adherent culture can well separate, purify and culture the BMSCs, and the obtained cells have better proliferative capacity, and the 3th -6th generations are more suitable for experimental research.关键词
骨髓基质干细胞/分离和提纯/细胞培养技术/大鼠,WistarKey words
Bone marrow stromal cell/Isolation & purification/Cell culture technique/Rat, Wistar分类
医药卫生引用本文复制引用
何玉祥,孙岩,刘振川,刘洋,周华,王茂华,袁海,金星,吴学君..大鼠骨髓基质干细胞体外分离、纯化、培养的实验研究[J].解放军医药杂志,2014,(5):10-13,4.基金项目
山东省自然科学基金(ZR2012HM063) （ZR2012HM063）
高等学校博士学科点专项科研基金(20090131110059) （20090131110059）
山东省优秀中青年科学家科研奖励基金项目(2005BS03007) （2005BS03007）
