摘要
Abstract
Objective To evaluate the clinical value of flow-through hybridization with genechip technology in detection of human papilloma virus ( HPV) of female genital tract, and to explore the most common HPV genotypes and high nosogenic age. Methods The cervical cells of 21 kinds of HPV genotyping assays were performed for 545 cervical cytology specimens using flow-through hybridization with genechip technology. The HPV-positive rates were compared a-mong normal cytology group ( n=120 ) , abnormal cytology group ( n=425 ) and abnormal types of abnormal cytology group 174 cases of chronic cervical inflammation, cervical intraepithelial neoplasia (102 cases of CIN I, 74 cases of CIN II and 36 cases of CIN III) , 16 cases of cervical carcinoma and 23 cases of condyloma acuminatum] . Abnormal cytology group was divided into 4 age subgroups:85 cases of the 20-31 age group, 140 cases of the 31-41 age group, 90 cases of the 41-51 age group and 110 case of older than 51 age group, and HPV infection rates in the four groups were com-pared. Results The HPV-positive rate was 22. 5% (27/120) in normal cytology group and 87. 3% (371/425) in ab-normal cytology group respectively. The differences in HPV-positive rates in abnormal cytology group and 6 subgroups in abnormal types of abnormal cytology group respectively compared with that in normal cytology group were statistically sig-nificant (P<0. 01). Six of the common HPV genotypes in normal cytology group were HPV16, 68, 18, 52, 58 and 11 in turn (by diminishing positive rate);six of the most common HPV genotypes in abnormal cytology group were HPV 16, 52, 58, 18, 33, 31. The HPV-positive rates in the 31-41 age and the 41-51 age groups were significantly higher than that in older than 51 age group (P<0. 01), the 31-41 age group had the highest HPV-positive rate, and the rate gradu-ally decreased with age increasing when the patients were 41 years old. Conclusion The flow-through hybridization with genechip technology may detect a variety of HPV infected subtypes and group types in just one test to improve the level of prevention and treatment of gynecologic cancer induced by HPV infection.关键词
基因芯片技术/乳头状瘤病毒感染/宫颈疾病/DNA指纹法Key words
Genechip technology/Papillomavirus infection/Uterine cervical disease/DNA Fingerprinting分类
医药卫生
