解放军医药杂志Issue(7)：4-7,20,5.DOI:10.3969/j.issn.2095-140X.2014.07.002
经皮置管引流减轻重症急性胰腺炎大鼠胰腺氧化应激损伤的研究
Percutaneous Catheter Drainage in Alleviation of the Oxidative Stress Damage in Rats with Severe Acute Panreatitis
摘要
Abstract
Objective To explore the influence of percutaneous catheter drainage (PCD) on the oxidative stress damage in rats with severe acute panreatitis (SAP). Methods Thirty Wistar rats were randomly divided into sham oper-ation group (n = 10) undergoing laparotomy, SAP group (n = 10) in which the SAP rat models were established by injec-ting Sodium Taurocholate regressed pancreas and bile ducts and PCD group (n = 10), which was added with PCD therapy based on the SAP treatment. Rats were killed 12 hours after the operation, and the levels of TNF-α, amylase, trypase and malondialdehyde (MDA) in serum and ascites were detected; paraffin sections of pancreatic tissues underwent patho-logical examination under light microscope, and contents of superoxide dismutase (SOD), myeloperoxidase (MPO) and MDA in pancreatic tissues were detected at the same time; the expression of (ADP-ribose)polymerase (PARP) in the pancreatic tissues was detected by Western Blot. Results Histopathological results and sections showed that the degrees of vacuolization, infection, oedema, necrosis and bleeding injury in SAP group were severe, compared with those in PCD group (P < 0. 05); the levels of TNF-α content and amylase activity in serum, contents of MPO and MDA in pancreatic tissues and PARP expression in SAP and PCD groups were significantly higher than those in sham operation group, and the values in PCD group were also significantly lower than those in SAP group (P < 0. 05); contents of TNF-α and MDA in ascites and trypase activity in PCD group were significantly decreased, compared with those in SAP group (P < 0. 05);the SOD contents in SAP and PCD groups were significantly lower than that in sham operation group, and the content in PCD group was higher than that in SAP group (P < 0. 05). Conclusion PCD can alleviate SAP-induced pancreatic oxi-dative stress damage by drainning harmful ascites, which contains inflammatory cytokine and trypsin.关键词
胰腺炎,急性坏死性/引流术/氧化应激/大鼠,WistarKey words
Pancreatitis, acute necrotizing/Drainage/Oxidative stress/Rat, wistar分类
医药卫生引用本文复制引用
陈光宇,陈振宇,戴睿武,陈涛,汪涛,吕润华,汤礼军..经皮置管引流减轻重症急性胰腺炎大鼠胰腺氧化应激损伤的研究[J].解放军医药杂志,2014,(7):4-7,20,5.基金项目
四川省科学事业计划基金资助项目(2011JTD0010) （2011JTD0010）
成都军区总医院院管课题(2013YG-B065) （2013YG-B065）
