解放军医药杂志Issue(7)：28-32,38,6.DOI:10.3969/j.issn.2095-140X.2014.07.008
携带 IL-2和 NK4双基因真核共表达载体的减毒沙门菌株的构建
Construction of an Attenuated Salmonella Typhimurium Containing IL-2 and NK4 Co-expressed Eukaryotic Vector
摘要
Abstract
Objective To construct an attenuated Salmonella typhimurium strain (TPIN) containing IL-2 and NK4 co-expressed eukaryotic vector. Methods A Eukaryotic expression vector (pCMV-IL-2-IRES-NK4) was construc-ted which contained IL-2 and NK4 genes by genetic engineering technology. The vector was transfected into cells using li-pofectamin and detected the expression levels of IL-2 and NK4 by enzyme linked immunosorbent assay (ELISA) and pol-ymerase chain reaction (PCR) methods. And then, the vector pCMV-IL-2-IRES-NK4 was conducted into an attenuated Salmonella typhimurium Ty21a by electrotransformation to build TPIN. Results The IL-2 / NK4 co-express vector steadi-ly expressed the IL-2 and NK4 genes and albumen with high levels by ELISA and PCR detection; the attenuated Salmo-nella typhimurium DNA vaccine with IL-2 / NK4 genes (TPIN) was constructed successfully. Conclusion An attenuated Salmonella typhimurium strain carrying IL-2 / NK4 genes has been successfully constructed.关键词
白细胞介素 2/沙门菌菌苗/基因/遗传载体Key words
Interleukin-2/Salmonella vaccines/Gene/Genetic vector分类
医药卫生引用本文复制引用
张尚弟,哈小琴,杨志华,李兵,邓芝云,姜东红,张俊,王剑锋..携带 IL-2和 NK4双基因真核共表达载体的减毒沙门菌株的构建[J].解放军医药杂志,2014,(7):28-32,38,6.基金项目
甘肃省科技重大专项(1302FKDA039) （1302FKDA039）
兰州市科技项目(2011-2-60) （2011-2-60）
全国博士后基金(20060390192) （20060390192）
