解放军医药杂志Issue(8)：89-91,95,4.DOI:10.3969/j.issn.2095-140X.2014.08.025
结核感染T细胞斑点试验在结核性胸腔积液诊断中的意义
Significance of Tuberculosis Infection T Cell-Spot Test in Diagnosis of Tuberculous Pleural Effusion
刘倩颖 1王心静 2林明贵3
作者信息
- 1. 100853 北京，解放军医学院研究生学院
- 2. 100091 北京，解放军309医院结核一科
- 3. 100091 北京，解放军309医院结核病研究室
- 折叠
摘要
Abstract
Objective To explore the significance of tuberculosis infection T cell-spot test for tuberculosis infec-tion ( T-SPOT. TB) in diagnosis of tuberculous pleural effusion. Methods A total of 58 inpatients with tuberculous pleu-ral effusion ( observation group) during June 2011 and December 2013 and 42 inpatients without tuberculous pleural effu-sion ( control group) at the same time were selected. Positive rate of effector T lymphocytes ( spots formation, SFC) re-leasing gamma interferon after antigenic stimulation in pleural effusion of the patients was detected by T-SPOT. TB reagent kit, and the Adenosine Deaminase ( ADA) and serum mycobacterium tuberculosis antibody ( TB-AB) examinations were also performed, and then values of sensitivity, specificity, positive predictive value, negative predictive value and the di-agnostic accuracy by the three kinds of methods were compared. Results SFC positive rate of T-SPOT. TB in observation group was significantly higher than that in control group (91. 4% VS 7. 14%;χ2 =71. 2, P<0. 01);values of sensitivi-ty, specificity, positive predictive value, negative predictive value and the diagnostic accuracy by T-SPOT. TB were sig-nificantly higher than those by ADA and TB-AB examinations ( P<0. 05 ) , while the values by ADA examination were significantly higher than those by TB-AB examination, except for the specificity value ( P <0. 05 ) . Conclusion T-SPOT. TB in detection of the specificity T cell numbers releasing γ-interferon in pleural effusion has higher sensitivity in diagnosis of tuberculous pleural effusion, and can be used as an auxiliary diagnostic method. T-SPOT. TB combined with ADA or TB-AB examination may improve the differential diagnosis rate.关键词
胸腔积液/结核/γ-干扰素/T细胞斑点试验/腺苷脱氨酶/结核杆菌抗体/临床实验室技术Key words
Pleural effusion/Tuberculosis/Gamma interferon/T cell-spot test/Adenosine deaminase/Tuber-culosis antibody/Clinical laboratory technique分类
医药卫生引用本文复制引用
刘倩颖,王心静,林明贵..结核感染T细胞斑点试验在结核性胸腔积液诊断中的意义[J].解放军医药杂志,2014,(8):89-91,95,4.
