解放军医药杂志Issue(9)：13-16,4.DOI:10.3969/j.issn.2095-140X.2014.09.004
丹参对体外培养大鼠成纤维样滑膜细胞bcl-2和bax表达的影响
Effect of Salvia Miltiorrhiza on Fibroblastic Synovial Cells of bax/bcl-2 Expressions of Culture in Rats in Vitro
摘要
Abstract
Objective To explore the effect of Salvia Miltiorrhiza on fibroblastic synovial cells of bax/bcl-2 ex-pressions and proliferation culture in rats in vitro. Methods A concentration of 1. 5 × 10 -5/ ml RSC-364 fibroblastic synovial cells of 100 ul cell suspension was added with the cell culture plate,and was cultivated for 24 h,and then differ-ent concentrations of Salvia Miltiorrhiza culture medium were added. Inhibition effect of Salvia Miltiorrhiza on fibroblastic synovial cell proliferation and its time-effect relationship were analyzed using MTT method;bcl-2 and bax expressions were detected using immunohistochemical method. Results Salvia Miltiorrhiza was able to inhibit the cell proliferation of fibroblastic synovial cells in rats,and it showed a certain degree of concentration-response and time-effect relationships. Salvia Miltiorrhiza could significantly increase the bax expression of fibroblastic synovial cells,and reduce bcl-2 expres-sion of synovial cells,and the differences,compared with those in control group,were statistically significant( P <0. 05). Conclusion Salvia Miltiorrhiza can inhibit the proliferation of fibroblastic synovial cell culture in rats in vitro , and its mechanism of action may relate to regulation of bax and bcl-2 expressions.关键词
丹参/成纤维细胞/细胞增殖/大鼠Key words
Salvia miltiorrhiza/Fibroblasts/Cell proliferation/Rats分类
医药卫生引用本文复制引用
任威铭,张葆花,吴承艳,佟书娟..丹参对体外培养大鼠成纤维样滑膜细胞bcl-2和bax表达的影响[J].解放军医药杂志,2014,(9):13-16,4.基金项目
江苏省自然科学基金资助项目（BK20131418）；江苏省高等学校大学生实践创新训练计划项目 （）
